Fig. 2

Overview on attachment patterns of human mesenchymal stromal cells (MSCs). a Human bmMSCs (n = 14 donors, black bars) or pMSCs (n = 10 donors, grey bars) were expanded to investigate their attachment to peptides P2–P17 as indicated. Attachment of MSCs to laminin-111 (LM) and fibronectin (FN) served as positive control (100 %), attachment to activated bovine serum albumin (aBSA) served as negative control (0 %). Attachment of human DF to the same substratum was tested in comparison to the MSCs (n = 5 donors, hatched bars). b Attachment of primary culture human MSCs to peptides P7, P14, P15, and P17 was tested prior to deep freezing (naïve MSCs) and compared to the same cells after cryopreservation and revitalization (cryo MSCs). Overall, naïve MSCs attached to the peptides at 43 ± 18 % mean efficacy compared to FN (=100 %). Cryopreservation caused a reduced overall attachment to these peptides of all MSCs investigated (19.2 ± 14.2 %). Thus, cryopreservation yielded a significant drop of attachment of human MSCs (n = 10, mean reduction 0.42 ± 0.20, p ≤ 0.0048)