Table 1 List of reagents employed: monclonal antibodies for exploring mesenchymal stromal cells by flow cytometry
CD | Isotype | Clone | Label | Source | Company | Dilution |
|---|---|---|---|---|---|---|
CD14 | IgG2A | MφP9 | FITC | Mouse | R&D Systems | 1:5 |
CD34 | IgG1 | 4H11 | PE | Mouse | BioLegend | 1:10 |
CD45 | IgG1 | MEM-28 | PE | Mouse | BioLegend | 1:5 |
CD73 | IgG1 | AD2 | PE | Mouse | BD Bioscience | 1:2.5 |
CD90 | IgG2A | Thy1-A1 | PE | Mouse | R&D Systems | 1:5 |
CD105 | IgG1 | SN6 | AF 488 | Mouse | ABD Serotec | 1:5 |
CD 271 | IgG1 | ME20.4-1.H4 | APC/AF 647 | Mouse | Miltenyi Biotec | 1:5 |
CD29 / ITGB1 | IgG1 | MAR4 | BV510 | Mouse | BD Bioscience | 1:10 |
CD49b / ITGA2 | IgG2A | 12Â F1 | PE-CF594 | Mouse | BD Bioscience | 1:10 |
CD49d / ITGA4 | IgG1 | 9Â F10 | BB515 | Mouse | BD Bioscience | 1.10 |
CD49f / ITGA6 | IgG2A | GoH3 | PE-CF594 | Rat | BD Bioscience | 1:10 |
ITGA7 | Polyclonal serum | ø | FITC | Rabbit | Biorbyt | 1:10 |