Fig. 3

Purification of cells by FACS. BDNF-GFP-expressing cells (a) and GFP-expressing control cells (b) were sorted by FACS after MACS. The analysis gates were set with reference to negative controls, resulting in a 99.9 % purity of all stained cells (Q3). Sorting gates were set as follows. Left panels: the gate (P1) was set for cell size and granularity of the cells. Dead cells and cell debris (shown in black) were excluded. Middle panels: the gate (P2) was set for single cell sorting. Cell doublets (shown in red) were excluded. Right panels show the stringent gate (P3) for the PSA-NCAM sort. BDNF brain-derived neurotrophic factor, FSC forward scattering, GFP green fluorescent protein, PSA-NCAM polysialylated neural cell adhesion molecule, SSC side scattering, SSEA-1 stage-specific embryonic antigen-1