Fig. 2

In vitro differentiation potential of CD146⁺ and CD146^– MSCs. Isolated MSCs were cultured in chondrogenesis or osteogenesis condition medium, and then treated with 10 ng/ml TNFα or 10 % synovial fluid from RA patients. The cells were stained with Safranin O a, b and Alizarin Red S c, d to detect the differentiation potential for chondrogenesis and osteogenesis, respectively. Quantitative data for chondrogenesis and osteogenesis are expressed as mean ± SEM compared with the nondifferentiation-negative control. *P <0.05. RASF synovial fluid from rheumatoid arthritis patients, TNFα tumor necrosis factor alpha