Fig. 2From: Treatment of periodontal intrabony defects using autologous periodontal ligament stem cells: a randomized clinical trialCell isolation, characterization and surgery. A The impacted third molar of patients was extracted and subjected to cell isolation and cell characterization for cell colony-forming ability and osteogenic/adipogenic differentiation. B The production of cell sheet/scaffold transplants and in vivo transplantation, including: (a) cell sheet formation; (b) Bio-Oss® particulates; (c) cell sheet/scaffold transplants; (d) exposure of bone defects; (e) placement of transplants; and (f) closure of the flapBack to article page