Prospective purification of perivascular presumptive mesenchymal stem cells from human adipose tissue: process optimization and cell population metrics across a large cohort of diverse demographics

Table 2 Potential clinical applications and the amount of adipose tissue required to provide sufficient numbers of prospectively purified perivascular stem cells to eliminate the need for ex-vivo expansion

Clinical use	Estimated number of cells required	Amount of fat needed
Tissue engineered tendon	1.5 million/cm	12 ml/cm
Tissue engineered mandible	10 million/cm	80 ml/cm
Cartilage for nasal reconstruction	20 million	160 ml
Scaphoid nonunion	25 million	200 ml
Tibial nonunion^a	40 million	320 ml
Total ear reconstruction	50 million	400 ml
Critical limb ischemia^a	2 million cells/kg	16 ml/kg
Graft versus host disease^a	0.5–13 million cells/kg	4.8–125 ml/kg
Graft versus host disease^a	0.5–13 million cells/kg	(repeat doses required)

ISSN: 1757-6512