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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Development of a two-step protocol for culture expansion of human annulus fibrosus cells with TGF-β1 and FGF-2

Fig. 8

Relative quantitative gene expression of annulus fibrosus (AF) putative markers at 10 and 14 days of cultivation in the five groups. The control group did not express the COL2A1/COL1A1 ratio. The gene expression for COL1A1, COL2A1, COL5A1, COL12A1, SFRP-2, and COL2/COL1 ratio were analyzed and compared to those at day 0 of cultivation. Data represent mean ± standard deviation (n = 2). AF cells maintained their phenotypes after passage. a Relative quantitative gene expression at 10 days of cultivation. b Relative quantitative gene expression at 14 days of cultivation. COL collagen, FGF fibroblast growth factor, GF growth factor, TGF transforming growth factor, SFRP secreted frizzled-related protein

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Stem Cell Research & Therapy

ISSN: 1757-6512