Fig. 1

Reduction of CD90 in MSCs. a Non-transduced mesenchymal stromal cells (MSC) and MSCs transduced with lentiviral particles expressing short hairpin (sh)RNA against CD90 (shRNA CD90 MSC) were analysed by flow cytometry. An accentuated decrease in CD90 expression is observed in shRNA CD90 MSC (thick line), whereas non-transduced MSCs (slim line) expressed high levels of CD90. The shaded histogram indicates staining with isotype control antibody. Representative histograms from dental pulp MSCs are shown. b Significant decrease of CD90 median fluorescence intensity (MFI) on shRNA CD90 MSCs when compared to non-transduced MSCs. (MFI = MFI marker – MFI isotype). Bar graphs represent the average mean fluorescence intensity as the median ± SD of CD90-FITC on cell lines used in this work; n = 7; ****p < 0.001. c Relative mRNA expression levels of CD90 in MSC, shRNA control MSC, and shRNA CD90 MSC had consistently low expression of CD90. Data are presented as mean ± SD of experiments performed in triplicate; *p < 0.05