Fig. 4
Inhibitory effect of ES-CM on Stat3 signaling in vivo. a Renilla luciferase (Rluc) imaging of activated Stat3. Representative animals injected with 2 × 105 4T1 (Fluc/GFP-pStat3/Rluc) cells after treatment with 4T1-CM and ES-CM for 48 h into the left and right of the fourth pair of mammary fat pads respectively. b Quantitative analysis of Rluc signal indicated decreased p-Stat3 level. **P < 0.01 vs 4T1-CM, n = 5. c Representative examples of the expression of Stat3 and pStat3 of tumor tissue by immunofluorescence staining. Scale bar represents 100 μm. d Quantitative analysis of pStat3 to total Stat3 expression. **P < 0.01 vs 4T1-CM, n = 3. CM conditioned medium, ES embryonic stem