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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: TGFβ-induced switch from adipogenic to osteogenic differentiation of human mesenchymal stem cells: identification of drug targets for prevention of fat cell differentiation

Fig. 4

Expression of osteogenic and adipogenic markers in hMSCs under conditions used for gene expression microarray analysis. Cells were incubated for 7 days in adipogenic differentiation medium containing 50 ng/ml BMP2 (B-7), 50 ng/ml BMP2 + 5 ng/ml TGFβ (BT-7), 50 ng/ml BMP2 + 500 μM IBMX (BI-7), and 50 ng/ml BMP2 + 5 ng/ml TGFβ + 500 μM IBMX (BTI-7). Untreated cells at day zero (C-0) were used as a control. a mRNA expression of the bone marker ALP (ALPL), relative to that of the housekeeping gene RSP27A. Results represent the mean and standard deviation of duplicate experiments. Both BI-7 (p < 0.05) and BTI-7 (p < 0.01) are significantly higher than B-7 and BT-7. b mRNA expression of the fat marker adiponectin (ADIPOQ), relative to that of the housekeeping gene RSP27A. Results represent the mean and standard deviation of duplicate experiments. BI-7 is significantly higher than both B-7 and BTI-7 (p < 0.01)

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Stem Cell Research & Therapy

ISSN: 1757-6512