Fig. 2

Effect of T-BST-75 on the testicular morphology of mice. a A magnified image of hematoxylin and eosin-stained testicular sections of T-BST-75 mice. Maximum depletion of Gc leaving behind only Sertoli cells in the evacuated tubules was observed. Black arrowhead marks the nucleus of Sertoli cells, red arrowhead marks the cytoplasm of Sertoli cells which was intact in T-BST-75 mice, green arrowhead marks the peritubular cells. i, ii and iii are testicular sections from three different mice. Scale bar: 20 μm. b Mean tubular diameter (under same magnification) of T-BST-75 mice as compared to T-DMSO control mice. A significant (p < 0.05) decrease in tubular diameter was observed in T-BST-75 mice. c Mean tubular circumference of T-BST-75 mice as compared to T-DMSO control mice. A significant (p < 0.05) decrease in tubular circumference was observed in T-BST-75 mice. d Survival rate of mice injected with various doses of busulfan and those treated with vehicle. Survival rate was found to be significantly (p < 0.05) reduced in IP-BST mice (n = 5). T-BST-25, T-BST-50, T-BST-75, and T-BST-100 denotes mice injected with 25 μg, 50 μg, 75 μg, and 100 μg of busulfan in each testes respectively. T-DMSO denotes mice injected with DMSO in testes. IP-BST denotes mice injected with busulfan, intraperitoneally