Fig. 3

Nonhuman primate BM CD34⁺ cell isolation. a Purity of isolated primate CD34⁺ cells. CD34⁺ cells were isolated via magnetic bead-based antibody affinity purification as described in Methods. (Left) Scatter-plot representation of purified CD34⁺ cell preparations. Forward scatter (FSC) and side scatter (SSC) were used to define gate MNC. (Right) (black curve) Negative control. The signal was detected using a fluorescently-labeled APC and isotype-matched monoclonal mouse IgG control antibody. (Red curve) Purified cells incubated with fluorescently-labeled APC mouse anti-nonhuman primate CD34 mAb. b Cell morphology of the freshly purified nonhuman primate CD34⁺ cells (20 ×)