Fig. 1From: Mitochondrial co-chaperone protein Tid1 is required for energy homeostasis during skeletal myogenesisUpregulation of Tid1 during induced differentiation of C2C12 myoblasts. a Mouse C2C12 cells were induced to differentiate into myotubes using differentiation medium (DMEM + 2% horse serum) from day 0 to day 3. b The crude cell extracts of the C2C12 cells undergoing induced differentiation were prepared and the expression level of proteins of interest was detected by immunoblot analyses. The blot was probed with antibody against GAPDH to confirm equal loading of the protein amount. The expression profile of Tid1 (DNAJA3) mRNA was analyzed by the GEO database of the NCBI (http://www.ncbi.nlm.nih.gov/geoprofiles/). Downregulation of Tid1 in the mitochondrial dysfunction (c) and muscular dystrophy mice model (d). *P < 0.05, ***P < 0.001Back to article page