Fig. 5

Analysis of the proliferative and metabolic properties of ADSCs derived from SSc patients. Proliferation and metabolism of adipose-derived stem cells from systemic sclerosis patients (ADSC-SSc) was decreased over 14 days compared to healthy control ADSCs (ADSC-N). a Cell metabolism was assessed by the Alamar blue assay on days 1, 3, 7, and 14. Cells were assessed at passage 1 (P1) to P4. ADSC-SSc displayed significantly less metabolic activity on days 3, 7, and 14 at all passages assessed (p < 0.01) (n = 6). b Cell proliferation was assessed by DNA quantification on days 1, 3, 7, and 14. Cells were assessed at P1 to P4. ADSC-SSc were significantly less proliferative on days 3, 7, and 14 at all passages assessed (p < 0.01) (n = 6). **p < 0.01