Fig. 6

Gene expression analysis of selected transcripts. pMSCs were cultured in the presence or absence of LL-37 for 2 days, and profiled by real-time PCR according to (a) LL-37 receptors (EGFR and FPRL-1) and (b) pro-inflammatory and anti-inflammatory factors (IL-10, IDO, TGF-β, Galectin-1, IL-6, IL-1β, and TNF). The relative fold values were obtained by the 2 − ΔΔCt method, using the median Ct value of untreated MSCs as a reference. Results are presented as mean of three experiments (biological replicates). *p <0.05. **p <0.01. EGFR1 epidermal growth factor receptor 1, IDO indoleamine 2,3-dioxygenase, IL-10 interleukin 10, IL-1β1 interleukin 1 beta, IL-6 interleukin 6, pMSCs human placenta-derived MSCs, TNF tumor necrosis factor