Fig. 2

Characterization of differentially regulated probe sets at day 14 of differentiation in the human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). a The differentially expressed (absolute fold change ≥ 2, false discovery rate (FDR)-corrected p value < 0.05) probe sets at day 14, called developmental probe sets were further characterized using the online tool ‘DAVID’. The gene ontology categories (GOs) belonging to biological processes (BPs) overrepresented among the up- and downregulated probe sets (p < 0.05) were further subcategorized into two classes: “developmental” and “non-developmental” GOs. The numbers of the overrepresented GOs for up- and downregulated genes in hESCs and hiPSCs are shown on the top of each bar. b, c Venn diagrams indicating the intersections for up- or downregulated developmental GOs, among hESCs and hiPSCs, respectively. d, e KEGG pathways associated with the up- or downregulated developmental probe sets, respectively. Numbers indicate the total number of probe sets (*FDR-corrected; p value < 0.05). f Overlap analysis of the well-annotated three germ layer-specific genes obtained from Partek Genomics Suite, showing overlap among the developmental genes. The total number of the deregulated probe sets is indicated on the top of each column