Fig. 6
Endothelial induced MESP1 progenitor cells enhanced vascular repair in critical limb ischemia mouse model. a Protocol of endothelial cell induction and transplantation in 3D GMs. b Representative images of the hindlimb from control, free cell (107), and cell (3 × 105) induced within microcryogels (Cell + GM) transplanted mice. Dates are indicated on the top. (n = 6 in each group). c Stacked bar graph showing the percentage distribution of the physiological status of treated ischemic limbs on day 28 post-implantation of vehicle and Cell + GM. d The table represents the physiological status of the ischemic limb on day 28 post-implantation of vehicle and Cell + GM. Values represent the percentage of limb salvage, necrosis, or loss. Parametric X 2 test: * p < 0.05. e Monitoring blood perfusion in ischemic hindlimb with fluorescence imaging. f Photographs showing new blood vessel formation across ligation sites of the ischemic hindlimb 28 days after treatment