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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs

Fig. 3

HPC/HSC-iPSCs showed unbiased differentiation potential into hematopoietic mature cells. a Embryonic bodies derived from early-passage mesenchyme-iPSCs and HPC/HSC-iPSCs; scale bars, 200 μm. b Representative hematopoietic colonies derived from iPSCs with Wright–Giemsa staining to reveal the hematopoietic progenitors. From left to right: BFU-E erythrocyte, M macrophage, GM granulocyte-macrophage, and GEMM granulocyte-erythrocyte-macrophage-megakaryocyte. Scale bars: top, 100 μm; bottom, 40 μm. c In vitro hematopoietic colony number per 50,000 cells of EBs differentiated from mesenchyme-iPSCs (n = 5) and hematopoietic-iPSCs (n = 4), using R1 cells as a control. The error bars indicate SD. d The comparison of CFU numbers between mesenchyme-iPSCs and hematopoietic-iPSCs (n = 3 measurements). Error bars indicate the SD P, P value, unpaired t test. EB embryonic body, HPC/HSC hematopoietic progenitor and stem cell, iPSC induced pluripotent stem cell, CFU colony-forming unit

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Stem Cell Research & Therapy

ISSN: 1757-6512