Fig. 3
From: CCL11 promotes migration and proliferation of mouse neural progenitor cells
Isolation of NPCs and migration assay. a The neurosphere from embryonic and infant mouse brains. Genes involved in proliferation and multipotency were expressed in cultured NPCs. Scale bar = 50 μm. b Flow-cytometric analysis for NPCs. The left panel showed an isotype control mouse IgM antibody. The right panel showed that PSA-NCAM was expressed in NPCs. c Immunostaining of Dcx for embryonic NPCs. Dcx-positive cells showed spindle cell morphology. Green: Dcx; blue: DAPI. Scale bar = 30 μm. d Migration assay using μ-Slide Chemotaxis. NPCs were seeded at 3 × 106 cells/ml in the shaded area. Chemokine was injected through the indicated port. Time-lapse imaging and tracking of NPCs with CCL11 (blue line). Scale bar = 100 μm. e, f The trajectory of randomly selected embryonic (e) and infant (f) NPCs in migration assay. Each color represents the trajectory of an individual cell. The y-axis and the x-axis represents migration distance (μm). The minus (leftward) direction of cell movement is defined as chemokine-induced migration. g The distance and end point of embryonic NPCs migration. CCL11 significantly increased the migration distance of NPCs compared with the control (PBS) and other chemokines. The data are presented as the mean of end points ± S.D. The migration assay was repeated three independent times per chemokine. * P < 0.05, ** P < 0.01. h The end point of trajectory of the embryonic NPCs (E-NPC) and infant NPCs (I-NPC) derived from the injury and intact side of mouse brain using CCL11. * P < 0.05. n.s. not significant