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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Laminin differentially regulates the stemness of type I and type II pericytes

Fig. 2

Laminin γ1 expression is abrogated in both type I and type II pericytes isolated from laminin γ1flox/flox:Pdgfrβ-Cre+(PKO) mice. a Diagram of type I and type II pericyte isolation procedure. b Representative plot of PDGFRβ-FMO control. c, d Representative plots showing sorting gates for PDGFRβ (c) and type I and type II pericytes (d). e Immunocytochemical analysis of laminin γ1 (red) expression in type I pericytes isolated from wild-type (WT) and PKO muscles. f Immunocytochemical analysis of laminin γ1 (red) and GFP (green) expression in type II pericytes isolated from WT and PKO muscles. g, h Quantitative data demonstrating the percentage of laminin-positive cells in type I (g) and type II (h) pericytes sorted from WT and PKO mice. n = 5. Data are shown as mean ± SD. ***p < 0.001 versus WT. Scale bars = 50 μm

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