Fig. 3From: Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cellsCryomicroscopic analysis of crystal formation during temperature fluctuations. Freezing of placental MSCs from 0 °C to –100 °C and thawing from –100 °C to –6.7 °C from left to right, top to bottom (the temperature is indicated on each image). a Samples at –80 °C during the first cycle of temperature fluctuations; b samples at –100 °C during the last cycle of temperature fluctuations; c samples at –80 °C during the last cycle of temperature fluctuations. Arrows indicate the change of inclusions. Scale bars = 20 μmBack to article page