| FACS Anexin V with PI | ||||
---|---|---|---|---|---|
Anexin V–,PI– (no necrosis or apoptosis), % | Anexin V–,PI + (necrosis), % | Anexin V+,PI– (apoptosis), % | Anexin V+,PI + (necrosis and apoptosis), % | ||
Constant cryopreservation temperature, –196 °C | 83.88 ± 3.7 | 1.02 ± 0.05 | 1.33 ± 0.89 | 13.7 ± 1.7 | |
Temperature fluctuations between –196 °C and –80 °C | 5 cycles | 82.7 ± 2.1 | 1.19 ± 0.03 | 2.21 ± 0.8* | 14.66 ± 2.0 |
10 cycles | 68.2 ± 2.1* | 1.12 ± 0.02 | 2.1 ± 0.09* | 27.98 ± 2.2* | |
20 cycles | 56.8 ± 1.5* | 0.54 ± 0.01 | 2.5 ± 0.08* | 41.47 ± 3.82* | |
30 cycles | 54.2 ± 3.1* | 1.01 ± 0.02 | 3.05 ± 0.09* | 42.68 ± 2.09* | |
40 cycles | 52.5 ± 1.8* | 0.72 ± 0.02 | 2.65 ± 0.15* | 43.64 ± 1.28* | |
50 cycles | 51.8 ± 1.9* | 0.86 ± 0.01 | 1.83 ± 0.05* | 46.22 ± 2.38* | |
Temperature fluctuations between –196 °C and –100 °C | 5 cycles | 81.6 ± 2.8 | 0.48 ± 0.02 | 1.67 ± 0.06 | 16.09 ± 1.44 |
10 cycles | 80.2 ± 2.9 | 0.58 ± 0.02 | 2.17 ± 0.07* | 17.47 ± 1.88* | |
20 cycles | 72.0 ± 2.5* | 0.89 ± 0.01 | 2.03 ± 0.02* | 26.05 ± 3.54* | |
30 cycles | 70.6 ± 3.1* | 1.18 ± 0.02 | 2.96 ± 0.05* | 24.11 ± 4.37* | |
40 cycles | 64.8 ± 2.4* | 0.65 ± 0.04 | 3.23 ± 0.01* | 25.96 ± 2.78* | |
50 cycles | 59.6 ± 3.3* | 1.6 ± 0.01 | 1.15 ± 0.01 | 38.94 ± 3.64* | |
Temperature fluctuations between –196 °C and –150 °C | 5 cycles | 88.37 ± 2.7 | 0.55 ± 0.01 | 1.94 ± 0.01 | 11.82 ± 3.02 |
10 cycles | 83.6 ± 1.8 | 0.83 ± 0.07 | 1.8 ± 0.09 | 13.64 ± 1.1 | |
20 cycles | 80.02 ± 3.2 | 0.57 ± 0.06 | 1.59 ± 0.03 | 17.68 ± 2.81 | |
30 cycles | 78.79 ± 4.5* | 0.34 ± 0.02 | 1.57 ± 0.03 | 19.30 ± 0.77* | |
40 cycles | 70.39 ± 4.1* | 0.98 ± 0.02 | 1.3 ± 0.03 | 25.46 ± 3.39* | |
50 cycles | 64.49 ± 3.6* | 0.96 ± 0.08 | 1.6 ± 0.05 | 27.74 ± 2.7* |