Fig. 5From: Hepatic population derived from human pluripotent stem cells is effectively increased by selective removal of undifferentiated stem cells using YM155Hepatic functional tests with stem cell iHeps pretreated withYM155 at stage II. WA01-iHeps pretreated with 5 nM YM155 at stage II showed the typical hepatic morphology, glycogen synthesis, LDL uptake and immunoreactivity of AFP and ALB (a). With stem cell iHeps (QIA7-iHeps and WA01-iHeps) pretreated by 5 nM YM155 selected as the optimal concentration at stage II, the amount of albumin (b) and urea (c) secreted for 24 h at day 22 of differentiation was measured and compared with DMSO control, HepG2 and p-Heps. Also, drug clearance was determined with aflatoxin B1 (1 μM) and acetaminophen (100 μM) (d). After each compound was incubated with QIA7-iHeps, WA01-iHeps and p-Heps for 24 h, the concentration of each compound was measured using the HPLC method (d). *p < 0.05 and different superscript letters indicate significant differences. AFP alpha-fetoprotein, ALB albumin, DMSO dimethyl sulfoxide, iHep induced hepatocyte, LDL low density lipoprotein, PAS periodic acid SchiffBack to article page