Fig. 5

Regulation of inflammatory mediators by human and feline ASCs differs. Feline ASCs in the presence of proliferating lymphocytes do not inhibit production of interferon gamma (IFNγ). Removal of direct contact between ASCs and stimulated peripheral blood mononuclear cells (PBMCs) led to a significant reduction in IFNγ production (a). Human ASCs in contact with PBMCs stimulated with leucoagglutinin (PHA) inhibit production of IFNγ. However, when contact with activated PBMCs is removed, inhibition of IFNγ does not occur (b). Feline ASCs inhibit production of tumor necrosis factor alpha (TNFα) with or without contact, with the transwell condition inhibiting production of the mediator to a greater extent (c). Human ASCs inhibit production of TNFα regardless of contact with proliferating lymphocytes (d). Production of interleukin-10 (IL-10) in feline ASC lymphocyte co-cultures increased significantly without contact and was also increased with contact (P = 0.06) (e). No alterations in IL-10 production were observed by activated human ASCs (f). Data presented as mean and standard error. *P < 0.05, **P < 0.01, ***P < 0.001. ConA concanavalin A, MSC mesenchymal stem cell