Fig. 6

Transcriptome analysis reveals similarities between human and feline adipose-derived mesenchymal stem cells (ASCs) at the molecular level. Total RNA was isolated from undifferentiated feline ASC primary cultures established from three different cats. Correlation coefficients (Pearson r) show the relatedness of the different feline ASC cultures as determined by pair-wise correlation analysis of the gene-level FPKM data (a). Substantial similarity of the feline ASC lines is demonstrated by the Venn diagram, which depicts a greater than ≥87% overlap in gene expression profiles (401 genes) based on all genes exhibiting a high level of expression (≥100 FPKM) (b). Functional analysis reveals prominent biological processes in feline MSCs. Gene annotation-enrichment analysis of the most highly expressed genes (≥100 FPKM) in common to the three ASC preparations was performed. The gene ontology and pathway annotations (biological processes and pathways) specifically enriched within this gene list were filtered for statistical significance (Fisher Exact P value ≤0.1), and selected, representative categories presented (c)