Fig. 4

DMM mice injected with PBS and HGP induced collagen type X expression in articular cartilage but HGP-CK2.1 did not. DMM mice injected with PBS or HGP-CK2.1 (6 μM) or HGP and sham-operated mice were immunostained for collagen type X (green), and Hoechst (blue) was used to determine the nucleus of the residing cell and location. Immunostaining demonstrates increased collagen type X expression in the AC of PBS- and HGP-injected mice but not HGP-CK2.1-injected mice. Scale bars = 100 μm. AC articular cartilage, HGP hydrogel particle, MF medial femur, MC marrow cavity, PBS phosphate-buffered saline, PC patellar cavity, SHAM sham-operated