Fig. 4

The hPDSC injection following point of time on a radiation-induced small intestinal damage model. a The animal experimental procedure. b On day 10 after irradiation, relative analysis measurements of weight of small intestine. Each value represents the average of eight independent measurements per group. c Histologic structures of radiation-induced intestinal damage following indicated groups. The estimated histological score by three independent blinded pathologists. Results are representative pathologic images of three independent samples (×100 magnification). d The expression of iNOS, COX2, and β-actin (as loading control) in protein extracts of each according to group measured with Western blotting. e The concentration of MDA in experimental groups. Results are presented as the mean and SD of triplicate values respectively. f The hPDSC injection extends life in an animal model of radiation-induced small intestine disorders: C57BL6 mice were subjected to lethal whole body irradiation at a 10 Gy dose. hPDSCs were administered by intravenous infusion. Controls received vehicle. Time points of interventions are given above the survival plots. P value determined by t test. g Mice, n = 8 in each group, were randomly sacrificed at 7 days after irradiation to obtain femur, lung, liver, and spleen for H&E staining (×400 magnification). Results are representative of eight independent samples. COX-2 cyclooxygenase-2, hPDSCs human placenta-derived mesenchymal stem cells, iNOS nitric oxide synthase, IR ionizing radiation