Fig. 4From: High quality clinical grade human embryonic stem cell lines derived from fresh discarded embryosHistological sections of teratomas derived from MAN lines 13–16. The teratomas were formed by subcutaneous injection of MAN13–16 cells under the kidney capsule of a SCID mouse. Stained sections show hematoxylin and eosin staining (A-L) of structures representative of the three germ layers: endoderm (A, D, G, J), mesoderm (B, E, H, K) and ectoderm (C, F, I, L) for MAN13 (A-C), MAN14 (D-F), MAN15 (G-I) and MAN16 (J-L). A, Kidney from endoderm; B, smooth muscle from mesoderm; C, retinal pigment epithelium from ectoderm; D, oesophagus from endoderm; E, artery from mesoderm; F, primitive neuroepithelium from ectoderm; G, intestine from endoderm; H, skeletal muscle from mesoderm; I, neural epithelium from ectoderm; J, sebaceous gland from endoderm; K, smooth muscle from mesoderm; L, skin tissue from ectoderm. a-d Alcian blue-fast red staining showing cartilage (mesoderm) in (a) MAN13, (b) MAN14, (c) MAN15 and (d) MAN16. Scale bars represent 100uMBack to article page