Fig. 5From: Interleukin-3 enhances the migration of human mesenchymal stem cells by regulating expression of CXCR4Comparison of effect of different cytokines on regulation of CXCR4. To compare the induction of CXCR4 expression by different cytokines, BM-MSCs and AT-MSCs were incubated with IL-1β (10 ng/ml), IL-3 (100 ng/ml), IL-17A (50 ng/ml) and TNF-α (50 ng/ml) independently for 24 hours. Fold change in percentage of CXCR4+ cells was analyzed by flow cytometry (a). Expression of MHC class I (b) and class II (c) molecules on MSCs was analyzed by flow cytometry after IL-3 treatment for 24 hours, and compared with respective untreated MSCs. Data shown as mean ± SEM of three independent experiments. *p ≤ 0.05 and **p ≤ 0.01 vs control groups. AT adipose tissue, BM bone marrow, CTRL control, CXCR4 chemokine (C-X-C motif) receptor 4, GT gingival tissue, IL-3 interleukin-3, MSC mesenchymal stem cellBack to article page