Fig. 7
In-vivo migration of IL-3-treated cells towards SDF-1α. Two matrigel plugs mixed with SDF-1α (100 ng/ml) were injected subcutaneously at the right dorsal side of NOD/SCID mice and two matrigel plugs without SDF-1α were injected on the left dorsal side of mice. BM-MSCs and AT-MSCs untreated or pretreated with IL-3 were labeled with Qtracker 655 and injected subcutaneously (105 cells in 100 μl) at the center, equidistant from all four implants. Schematic representation of the subcutaneously implanted matrigel in mouse model (a). Mice were acquired on the Live Cell Imaging System at 0 hours for detection of labeled MSCs and at 24 hours for detection of migrated MSCs towards the matrigel plugs (b, c). Graphical representation showing the counts of fluorescent intensity at the region of interest (ROI), the area of matrigel plugs (d). Difference between in-vivo migration of IL-3-treated and untreated MSCs towards SDF-1α (BM-IL-3-S vs BM-S and AT-IL-3-S vs AT-S) was compared. Matrigel plugs were harvested from mice and isolated cells were acquired on flow cytometry (e). C matrigel implant without SDF-1α, S matrigel implant containing SDF-1α. Data shown as mean ± SEM (mice, n = 6 and matrigel plugs, n = 12/group). *p ≤ 0.05 and ***p ≤ 0.001 vs control groups. AT adipose tissue, BM bone marrow, IL-3 interleukin-3, MSC mesenchymal stem cell, SDF-1α stromal cell-derived factor-1 alpha