Fig. 5

NAMPT or SIRT2 inhibition led to diminished deacetylation of p53 with subsequent upregulation of p21 expression in human iPS cells. a Western blot analysis of total p53 and acetyl-K382 p53 protein expression in human iPS cells treated with FK866, AC93253, or DMSO for 48 h. GAPDH was used as loading control. Representative WB images are depicted. Diagrams show acetylated p53 to total p53 protein ratio in arbitrary units (AU). Data represent means ± SD from two independent experiments, each in duplicates (*p < 0.05, **p < 0.01, ***p < 0.001). b mRNA expression of p21 in human iPS cells treated with FK866, AC93253, or DMSO for 48 h. Fold changes relative to DMSO-treated cells are shown. Data represent means ± SD from two independent experiments, each in triplicates (*p < 0.05, **p < 0.01, ****p < 0.0001). c Western blot analysis of p21 protein expression in human iPS cells treated with FK866, AC93253, or DMSO for 48 h. GAPDH was used as loading control. Representative WB images are depicted. Diagrams show p21 to GAPDH protein ratio in arbitrary units (AU). Data represent means ± SD from two independent experiments, each in duplicates (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). d Representative WB images of corresponding protein expression in human iPS cells transduced with the indicated shRNA constructs