Fig. 4

In-vitro ABCB5⁺ MSCs restore ER Ca²⁺ using the SERCA2a-PLN axis. A The representative western blot showing SERCA2a, PLN, pPLN and β-actin expression in HUVECs under different treatments. B–D HUVECs were treated with 80 μM CoCl₂, or 120 μM DFO or 100 μM H₂O₂ for duration of 4 h. Afterward, HUEVCs were either co-cultured with ABCB5⁺ MSCs (CoCl₂-CO; DFO-CO) or cultured in ABCB5⁺ MSC-CM (CoCl₂-CM; DFO-CM) for 24 h. The expression of SERCA2a, PLN, Phosphorylated PLN (pPLN) and β-actin expression was determined by western blotting and quantified by densitometry using NIH ImageJ 1.52 software. Expression of SERCA2a and PLN is presented as fold change compared to untreated control cells while pPLN is shown as ratio of pPLN/PLN. Quantitative analysis of B SERCA2a, (normalized to β-actin) and C PLN expression (normalized to β-actin); and D ratio pPLN to PLN