Fig. 7

Immunofluorescent staining for angiogenesis in the IBZ. a Immunofluorescent staining for the laminin-positive area to evaluate angiogenesis in the IBZ. Scale bar = 100 μm. b ANOVA detected significant treatment effects on angiogenesis as assessed by measuring the percentage of laminin-positive area, which significantly increased in the SB623 + Ex group compared to the vehicle, Ex, and SB623 groups, respectively (each group: n = 5, F_{(3, 16)} = 14.0, p < 0.001). The Ex group and the SB623 group had significantly increased in laminin-positive percentage areas in the IBZ compared to the vehicle group (mean ± SE, ^*p < 0.05, ^**p < 0.01 vs. vehicle group, ^#p < 0.05 vs. SB623 + Ex group). Abbreviation: IBZ: ischemic boundary zone