Fig. 3

Effect of YAP on in vitro differentiation of human iPSCs to hematopoietic stem cells. A Schematic diagram of the protocol used to generate HSPCs from iPSCs in vitro. B Morphologies of differentiated EBs derived from control, YAP-S5A and YAP-KD cell lines on day 4, 8 and 10 after being plated on Matrigel. The yellow arrow indicates Hematopoietic Stem Cells (HSCs), black arrow indicates Hemogenic cells Endothelial (HE). C Histogram showing the number of, D total counts of floating and adherence cells Percentage of HSPCs, and E Number of HSPCs harvested from both floating and adherent cells at day 10 of culture. Each dot represents individual replication. All experiments were performed at least three times independently with the technical triplicate, and data were expressed as mean ± SEM, Mann–Whitney U test, the p-value of ***p < 0.001, **p < 0.01, *p < 0.05