Table 1 Primer sequences used in the quantitative RT-PCR
Gene | F (5′–3′) | R (5′–3′) |
|---|---|---|
FOXA2 | AGCGCCCACGTACGACGACATG | AGAGCCCGAGGGCTACTCCT |
Sox17 | TCCCTACCAGGGACACGACT | GAGCTAGCGTCGGACACCAC |
PDX1 | GTCCAGCTGCCTTTCCCAT | TCCGCTTGTTCTCCTCCG |
Ngn3 | GGAGTCGGCGAAAGAAGG | AAGCTGTGGTCCGCTATGC |
NKx6.1 | TTGGACAAAGACGGGAAGAG | CTGTCATCCCCAACGAATAG |
Insulin | GGGAACGAGGCTTCTTCTACA | ACAATGCCACGCTTCTGC |
GLUT2 | TCCAGCTACCGACAGCCTATT | GCACAAACAAACATCCCACTCA |
MAFA | TTCAGCAAGGAGGAGGTCAT | CCGCCAGCTTCTCGTATTTC |
GAPDH | AATCCCATCACCATCTTCCA | TGGACTCCACGACATACTCA |