A new generation of mesenchymal stromal/stem cells differentially trained by immunoregulatory probiotics in a lupus microenvironment

Table 1 Effects of treatments on kidney, spleen, liver, and body weight of mice at the end of the study

Groups	Kidney (gr)	Spleen (gr)	Liver (gr)	Bodyweight (gr)
N–C	0.156 ± 0.0073	0.1340 ± 0.005	1.164 ± 0.005	27.96 ± 0.1958
P–C	0.158 ± 0.0047	0.5160 ± 0.0269	1.594 ± 0.035	29.72 ± 0.4164
N-MSCs	0.155 ± 0.0041	0.2520 ± 0.012	1.660 ± 0.0594	29.34 ± 0.3586
R-MSCs	0.156 ± 0.0086	0.1680 ± 0.0139	1.464 ± 0.0320	28.96 ± 0.2839
D-MSCs	0.157 ± 0.0015	0.1600 ± 0.0070	1.522 ± 0.0354	28.92 ± 0.3813
DR-MSCs	0.156 ± 0.0097	0.1660 ± 0.0120	1.476 ± 0.0581	29.10 ± 0.2864
Groups	P value for the source of variation
P–C vs N–C	0.3531 (ns)	0.0021 (***)	0.0018 (**)	0.1391 (ns)
P–C vs N-MSCs	0.6954 (ns)	0. 0075 (**)	0.8628 (ns)	0.3063 (ns)
P–C vs R-MSCs	0.4136 (ns)	0.0051 (**)	0.0635 (ns)	0.7007 (ns)
P–C vs D-MSCs	0.6219 (ns)	0.0022 (**)	0.8710 (ns)	0.2021 (ns)
P–C vs DR-MSCs	0.9989 (ns)	0.0010 (**)	0.7541 (ns)	0.8277 (ns)

The statistical significance was determined by One-way analysis of variance (ANOVA). Tukey’s multiple comparisons were used to determine the relationship between the variable’s means. N–C: Negative Control (Healthy mice treated with PBS); P–C: Positive Control (Pristane-immunized mice treated with PBS); N-MSCs (Naïve MSCs without any interventions); R-MSCs: MSCs exposed to lactobacillus rhamnosus; D-MSCs: MSCs exposed to lactobacillus delbrueckii; DR-MSCs: MSCs exposed to a mixture of lactobacillus rhamnosus and delbrueckii. Data were presented as Mean ± Standard Error of the Mean (SEM). P values of ≤ 0.05 were considered significant. (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001)

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