Fig. 1
From: Vitamin C facilitates direct cardiac reprogramming by inhibiting reactive oxygen species
Forced expression of Gata4, Mef2c and Tbx5 converts mouse embryonic fibroblasts (MEFs) into iCMs. A Strategy for creating a direct cardiac reprogramming system with MEFs containing an αMHC-GFP+ reporter. MEFs carrying a stable Doxy-inducible TetO promotor, mouse GMT reprogramming factors and a mCherry tag, can be converted into induced cardiomyocytes (iCM) upon exposure to Doxy. B Relative mRNA expression of GMT during reprogramming in GMT-transduced MEFs. RNA samples were collected from three independent experiments. C Representative fluorescent images of GFP expression in MEFs after Doxy exposure. Scale bar 200 μm. D, Representative FACS images of GFP+ MEFs upon Doxy exposure. Upon exposure of Doxy, GFP expression is induced in MEFs while not being present in non-exposed MEFs. Data were analyzed with t-test. ****p ≤ 0.0001 vs control. (Doxy: Doxycycline)