Fig. 3
From: Vitamin C facilitates direct cardiac reprogramming by inhibiting reactive oxygen species
Analysis of differential expression upon GMT reprogramming and VitC exposure by RNA-Seq in MEFs. A PCA plot analysis was based on 622 differentially expressed genes between Doxy exposure alone and Doxy plus VitC (FDR = 0.1). The first principal components represent 95% of the variance in the RNA-seq data which indicated that the variance primarily comes from the difference between these two groups. C, E, G, heatmaps demonstrated that in muscle cell development pathway, genes are significantly upregulated in Doxy plus VitC group as compared with Doxy exposure alone, while in histone methylation and acetylation pathway, genes are significantly downregulated in Doxy plus VitC group (FDR = 0.1). B, D F, GSEA was performed to elucidate whether the applied gene set is statistically enriched in certain pathways, such as muscle cell development, histone methylation and acetylation pathway as indicated above. NES = 1.65, − 1.68 and − 1.42, respectively, FDR < 0.1. NES, normalized enrichment score. H, Enrichment analysis revealed several significant pathways in RNA-seq. Data were analyzed through Qlucore Omics Explorer and the package “EnrichR” enriched for “GO_Biological_Process_2015” was used. (Doxy: Doxycycline)