Fig. 6
From: Vitamin C facilitates direct cardiac reprogramming by inhibiting reactive oxygen species
VitC enhances direct conversion of cardiomyocytes from hFCFs via ROS suppression. A Schematic overview of direct cardiac reprograming in hFCFs. B Representative FACS images of α-actinin+ hFCFs upon Doxy exposure. C Statistical analysis of α-actinin+ hFCFs upon Doxy exposure. D Relative mRNA expression of cardiac genes ACTN2 and MYH6 seven days after reprogramming. E Schematic overview of direct cardiac reprograming in spheroid derived from hFCFs. F, G Representative FACS images and statistical analysis of α-actinin+ spheroid derived from hFCFs. H Chemical structure of ascorbic acid and dehydroascorbic acid. I Flow cytometry analysis of DCFDA detection to measure MEFs ROS production upon Doxy exposure, treated with ascorbic acid or dehydroascorbic acid. J Quantification of GFP+ MEFs treated with ascorbic acid or dehydroascorbic acid. K Flow cytometry analysis of DCFDA detection to measure hFCFs ROS production upon Doxy exposure, treated with ascorbic acid or dehydroascorbic acid. L Quantification of α-actinin+ hFCFs upon Doxy exposure, treated with dehydroascorbic acid. Mean values + SEM of three independent experiments is shown (n = 3). Data were analyzed with two-way ANOVA. *p ≤ 0.05 vs Doxy, ****p ≤ 0.0001 vs Doxy, ##p ≤ 0.01 vs control. (Doxy: Doxycycline)