Table 1 Primers list used for qPCR experiments
From: Vitamin C facilitates direct cardiac reprogramming by inhibiting reactive oxygen species
Genes | Forward primer | Reverse primer |
|---|---|---|
Human genes | ||
GATA4 | CGACACCCCAATCTCGATATG | GTTGCACAGATAGTGACCCGT |
MEF2C | CCAACTTCGAGATGCCAGTCT | GTCGATGTGTTACACCAGGAG |
TBX5 | CTGTGGCTAAAATTCCACGAAGT | GTGATCGTCGGCAGGTACAAT |
ACTN2 | CAAACCTGACCGGGGAAAAAT | CTGAATAGCAAAGCGAAGGATGA |
MYH6 | GCCCTTTGACATTCGCACTG | GGTTTCAGCAATGACCTTGCC |
P53 | GAGGTTGGCTCTGACTGTACC | TCCGTCCCAGTAGATTACCAC |
β-Actin | GATCGGCGGCTCCATCCTG | GACTCGTCATACTCCTGCTTGC |
Mouse genes | ||
Gata4 | TCAACCGGCCCCTCATTAAG | GTGGTGGTAGTCTGGCAGT |
Mef2c | ATCCCGATGCAGACGATTCAG | AACAGCACACAATCTTTGCCT |
Tbx5 | GGCATGGAAGGAATCAAGGTG | TTTGGGATTAAGGCCAGTCAC |
P53 | CTCTCCCCCGCAAAAGAAAAA | CGGAACATCTCGAAGCGTTTA |
β-Actin | GTGACGTTGACATCCGTAAAGA | GCCGGACTCATCGTACTCC |