Fig. 1
Human pluripotent stem cell-derived mesenchymal stem cells (pMSCs) reveal transcriptionally distinct populations. A Derivation of pMSCs from human brain-specific organoids. B Representative micrographs showing the typical cell morphology observed during the differentiation of hPSC to pMSCs. a: Day 0: hPSC colonies prior to differentiation; b: Differentiation of hESC into EB; c: human brain-specific organoids; d: pMSCs derived from hPSCs; scale bar, 200 µm. C Multilineage differentiation of pMSCs. After 2 weeks of corresponding induction, differentiated pMSCs were stained for mineralization with Alizarin Red, for chondrocytes with Toluidine Blue, or for lipid drops with Oil Red. D Percentage of surface markers in H1-MSCs, hiPSC-MSCs, and UC-MSCs. E Heatmaps showing genes related to pluripotency and MSC that were differentially expressed in H1-hESCs, hiPSCs, H1-MSCs, hiPSC-MSCs, and UC-MSCs. F Venn diagram and GO term analysis showing the number of common and distinct upregulated genes in the H1-MSCs and hiPSC-MSCs compared with UC-MSCs. G Heatmaps showing genes related to axon development and neural crest cell differentiation that were differentially expressed in H1-MSCs, hiPSC-MSCs, and UC-MSCs. H FACS analysis of surface markers of CD271 and CD73 in pMSCs from P0 to P3