Fig. 2

pMSCs support ex vivo maintenance and CFC expansion of UCB-CD34⁺ HSPCs. A Representative FACS analysis of UCB-CD34⁺ cells cocultured with and without H1-MSCs, hiPSC-MSCs, and UC-MSCs in 5% serum without added cytokines or SFEM medium with SCF, TPO, and FLT-3L for 7 days. B Cell yield of CD34⁺ Lin⁻ recovered from day 7 cocultures in 5% serum without added cytokines or SFEM medium with SCF, TPO, and FLT-3L. C, D Cell yield of total number of cells, CD34⁺CD38⁺ Lin⁻, CD34⁺CD38⁻Lin⁻ from days 7–35 after coculture with and without H1-MSCs, hiPSC-MSCs, and UC-MSCs, respectively. E, F CFC assay results of UCB CD34⁺ HSCs following 7 or 35 days coculture with and without H1-MSCs, hiPSC-MSCs, and UC-MSCs, respectively. Morphology of hematopoietic colonies after 14 days of culture in semisolid culture media based on methylcellulose at 37℃ and 5% CO2. G Quantification of apoptosis of hematopoietic cells on 4 culture conditions by AnnexinV/PI staining. The data in the bar graphs in panels B–G represent the mean ± SD. N = 3–4 replicates