Fig. 3

pMSCs improve human hematopoietic cell engraftment in NDG mice. A Scheme of the experiment design. Human cord mononuclear cells were cocultured with and without pMSCs and UC-MSCs for 2 weeks and transplanted into sublethally irradiated BNDG mice. Flow cytometry analysis was used to detect the chimerism of human blood cells in the recipient mice's peripheral blood or bone marrow at 3 or 6 months post-transplantation. B Representative flow plots of human cells (hCD45⁺) engraftment in PBMC of BNDG mice 3 months post-transplantation with human mononuclear cells cocultured with and without UCB-MSCs or pMSCs. C Engraftment (percent of human CD45⁺ cells) of mice 3 months after transplants. D Representative flow plots of human cells (hCD45⁺) engraftment in PBMC of BNDG mice 6 months post-transplantation with human mononuclear cells cocultured with and without UCB-MSCs and pMSCs. E, F Engraftment (percent of human CD45⁺ cells) and multilineage differentiation (CD33⁺ myeloid cells and CD3⁺T cells) of mice 6 months after transplants. The data in the bar graphs in panels C, E, F represent the mean ± SD. N = 4–6 replicates