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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mesenchymal stem/stromal cells from human pluripotent stem cell-derived brain organoid enhance the ex vivo expansion and maintenance of hematopoietic stem/progenitor cells

Fig. 5

Wnt inhibitors of SFRP1 and SFRP2 are highly expressed in pMSCs and UCB-CD34+ cocultured with pMSCs. A Workflow of the experiment design with RNA and proteomic sequencing. Transcriptome of pMSCs and UC-MSCs were compared using RNA-seq analysis. Culture medium supernatant from pMSCs and UC-MSCs were compared by proteomic sequencing analysis. Transcriptome of UCB-CD34+ cells cocultured with and without MSCs for 7 days were compared using RNA-seq analysis. B Heatmaps showing genes related to negative regulation of Wnt signaling pathway were differentially expressed in H1-MSCs, hiPSC-MSCs, and UC-MSCs. C FPKM of SFRP1, SFRP2, and IGFBP2 expressed in H1-MSCs, hiPSC-MSCs, and UC-MSCs. D Venn diagram and GO term analysis showing the number of common and distinct upregulated proteins in culture medium supernatant of H1-MSCs and hiPSC-MSCs compared with UC-MSCs. E Heatmaps showing genes coupregulated in H1-MSCs and hiPSC-MSCs both in RNA-seq and proteomics analysis. F Volcano Plot showing SFRP1 and SFRP2 upregulated in UCB CD34+ cells cocultured with H1-MSCs or hiPSC-MSCs compared that with UC-MSCs for 7 days. The data in the bar graphs in panels B represent the mean ± SD. N = 3–4 replicates

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Stem Cell Research & Therapy

ISSN: 1757-6512