Fig. 6

pMSCs antagonize Wnt signaling pathway activation and modulate the cell cycle of UCB-CD34⁺ cells. A Scheme of the experiment design. UCB-CD34 + cells transwell cocultured with and without pMSCs and UC-MSCs by addition of 3 µM CHIR99021 or DMSO. B–D CD34 + Lin-, CD34⁺ CD38⁻ Lin⁻, and Lin⁺ cell numbers after activation of Wnt signaling by addition of 3 µM CHIR99021 and DMSO. E Representative FACS analysis of CD34⁺ Lin⁻ cells at day 7 after coculture with and without H1-MSCs, hiPSC-MSCs, and UC-MSCs with addition of 3 µM CHIR99021 or DMSO. F The percentages of live, early apoptotic (E-apoptosis), late apoptotic (L-apoptosis), and dead cells in CD34⁺Lin⁻ cells at day 7 after coculture with and without H1-MSCs, hiPSC-MSCs, or UC-MSCs after activation of Wnt signaling by addition of 3 µM CHIR99021. G Representative flow cytometric analysis and quantification of BrdU incorporation and 7-AAD staining to determine the cell cycle distribution of 34⁺Lin⁻ HSPCs on day 7 of transwell culture after activation of Wnt signaling by addition of 3 µM CHIR99021. The data in the bar graphs in panels B–G represent the mean ± SD. N = 3–4 replicates