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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Uncovering impaired mitochondrial and lysosomal function in adipose-derived stem cells from obese individuals with altered biological activity

Fig. 5

Analysis of metabolites and enzymes in glycolysis and TCA in L-ADSCs and O-ADSCs. a Heatmap of cellular metabolites in O-ADSC versus L-ADSCs. b The concentration of glucose uptake and lactate secretion levels in the supernatant in L-ADSCs and O-ADSCs after culture for 24 h (n ≥ 3). c ECAR in L-ADSCs and O-ADSCs and qualification of ECAR processes. d OCR in L-ADSCs and O-ADSCs and their qualification in OCR processes. e ATP levels in the supernatant in L-ADSCs and O-ADSCs after culture for 24 h (n ≥ 3). f, g Western blot analysis of the protein levels of glycolysis related enzymes and TCA related enzymes and their qualification of relative protein intensity. Full-length blots are presented in Additional file 2: Figure S4 and S5

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