Fig. 1
A Steps of donor corneoscleral limbal tissue processing and isolation of anterior limbal stroma. B Tissue digestion—cell viability by trypan blue dye exclusion assay for freshly isolated cells after the pooled limbal stromal tissue digested with collagenase A (Collgn A, research-grade) from Roche, and NB6 (GMP collagenase I from Nordmark) were insignificantly different (ns). A significant drop in cell viability was observed with Celase (GMP collagenase from Worthington) digestion (*P < 0.01, One-way ANOVA). C Cell attachment profile by xCELLigence revealed similar efficiency between FNC and hFn coating. Normalized impedance readings were extracted at (a) 8 and (b) 24 h. Similar attachment efficiencies of CSSCs were found on FNC and hFn pre-coated surface (ns—not significant difference). CSSC attachment was suboptimal on hLn-521 and non-coated surface (*P < 0.05, One-way ANOVA)