Fig. 2

Optimizing GMP formulation of CSSC culture. A Growth kinetic profiles of donor CSSC batches in culture with JM-H medium with each research-grade component replaced by the corresponding GMP alternative. The numbers 570 and 584 represent donor corneal sources of CSSCs. Cell index profiles show the cell growth response up to 120 h. Blue arrows indicate 24-h intervals. The occasional interruptions are due to the pause for medium changes. The selected GMP substitutes showed similar culture outcomes as the research-grade reagents. B Percentages of cell growth recorded at 72 h show similar culture outcomes between each pair of GMP- and research-grade chemicals in CSSC propagation. *At the top profile indicates significant growth reduction of CSSCs cultured in DMEM/F12 (high L-glucose level) when compared to the mix of DMEM (low glucose) and MCDB201 in the laboratory-based JM-H medium (P < 0.05, one-way ANOVA). C Cell index profiles of CSSCs grown in the full GMP-compliant culture medium compared with the laboratory-based JM-H medium. D Cell doubling time at log phase illustrated similar CSSC growth in both media