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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Good manufacturing practice production of human corneal limbus-derived stromal stem cells and in vitro quality screening for therapeutic inhibition of corneal scarring

Fig. 3

Comparative assessment of cryopreservation media for cultured CSSCs. A Cell attachment efficiency—thawed cells after one month of frozen storage in different cryopreservation media was seeded on hFn-coated culture surface. After 24 h, the attachment rates of cells stored in CryoStor DMSO 5% and Cryopres DMSO 5% were similar to the research-grade DMSO[Res] (5%). Cell frozen in DMSO-free Stem-CellBanker, CryoStor DMSO 10%, and Cryopres DMSO 10% had poor cell attachment. B Cell index profiles of thawed CSSCs by xCELLigence. Cells in CryoStor DMSO 5% exhibited better growth kinetics. C Apoptosis assay by Annexin V-PI staining showed the percentages of live cells after frozen storage in 5% DMSO[Res], CryoStor DMSO, 5% and Cryopres DMSO 5% were similar

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Stem Cell Research & Therapy

ISSN: 1757-6512