Fig. 5

Keratocyte differentiation of CSSC under JM-H versus GMP cultures CSSC at P3 were cultured in a serum-free cytokine-supplemented condition (with bFGF and TGFβ3) for 7 days. A Typical dendritic morphology of keratocytes after induction. Scale bars: 50 µm. B Marked expression of keratocan (red) and aquaporin 1 (green) in the cytoplasm of differentiated cells. Scale bars: 50 µm C Similar response of laboratory-based and GMP-cultured CSSC in the expression of keratocyte markers, AQP1, B3GnT7, CHST6 and Lum. *P < 0.01; **P < 0.05, compared to undifferentiated CSSC; one-way ANOVA, nonparametric